This comprehensive guide covers the ins and outs of antibody conjugation, including the diference between direct and indirect assays, common labels used, diferent chemistries behind antibody labeling, and methods you can use to conjugate your primary antibody.
Exploring the next generation of antibody–drug conjugates
Jan 8, 2024 · Antibody–drug conjugates (ADCs) are a promising cancer treatment modality that enables the selective delivery of highly cytotoxic payloads to tumours.
Enzyme Conjugation - A Promising Tool for Bio-catalytic and ...
Jul 4, 2024 · Enzyme conjugation represents a cutting-edge facet of multi-enzyme biocatalysis. This technique primarily serves as a practical method for synthesizing two or more enzymes in a single cell, enabling co-purification or whole-cell bio-transformations.
Enzyme-Based Labeling Strategies for Antibody–Drug Conjugates …
Jan 4, 2018 · This review provides a general overview and focuses on chemo-enzymatic methods including enzymes such as formylglycine-generating enzyme, sortase, and transglutaminase. Applications for the conjugation of antibodies and antibody mimetics are reported.
Conjugation of enzymes to antibodies - PubMed
Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor ….
Cell–drug conjugates - Nature Biomedical Engineering
Jul 1, 2024 · Biomedical engineering. Cell delivery. Drug delivery. Drug development. Immunotherapy. Abstract. By combining living cells with therapeutics, cell–drug conjugates can potentiate the functions of...
A comprehensive overview on antibody-drug conjugates: from the ...
Sep 18, 2023 · Antibody-Drug Conjugates (ADCs) represent an innovative class of potent anti-cancer compounds that are widely used in the treatment of hematologic malignancies and solid tumors.
Enzymatic Bioconjugation: A Perspective from the Pharmaceutical ...
May 4, 2023 · To understand the role of conjugation site, we developed an enzymic method for site-specific antibody drug conjugation using microbial transglutaminase. This allowed us to attach diverse compds. at multiple positions and investigate …
Conjugation of Enzymes to Antibodies - Winston - 2000 - Current ...
May 1, 2001 · Conjugation of enzymes to antibodies involves the formation of a stable, covalent linkage between an enzyme [e.g., horseradish peroxidase (HRPO), urease, or alkaline phosphatase] and an antigen-specific monoclonal or polyclonal antibody in which neither the antigen-combining site of the antibody nor the active site of the enzyme is functionally ...
Enzymatic Bioconjugation: A Perspective from the Pharmaceutical ...
May 5, 2023 · However, available catalysts face stiff competition from other bioorthogonal chemistries. In this Perspective, we seek to illuminate applications of enzymatic bioconjugation in the face of an expanding palette of new drug modalities.