Phosphoglycolate has profound metabolic effects but most …
WEBFeb 2, 2019 — These 3′-phosphoglycolate groups can be removed either by releasing phosphoglycolate (via the enzyme APE1, [ 2 – 4 ]) or by releasing glycolate (via the enzyme TDP1, [ 5 – 7 ]) (see Supplementary Figure S1A). Phosphoglycolate is known to have an effect on two steps in glycolysis.
An essential developmental function for murine phosphoglycolate ...
WEBOct 12, 2016 — The free radical-initiated fragmentation of the DNA 2-deoxyribose sugar moiety generates single and double strand DNA breaks with a 3′-phosphate or a 3′-phosphoglycolate (3′-PG) terminus...
Processing of 3′-Phosphoglycolate-terminated DNA Double …
WEBFeb 9, 2007 — To assess the possibility that Artemis acts on oxidatively modified double strand break termini, its activity toward model DNA substrates, bearing either 3′-hydroxyl or 3′-phosphoglycolate moieties, was examined.
Conversion of Phosphoglycolate to Phosphate Termini on 3′ …
WEBFractionated human whole-cell extracts contained an activity, which in the presence of EDTA, catalyzed removal of glycolate from phosphoglycolate at a single-stranded 3′ terminus to leave a 3′-phosphate, reminiscent of the …
Deficiency in 3′-phosphoglycolate processing in human cells with …
WEBJan 12, 2005 — Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that removes peptide fragments linked through tyrosine to the 3′ end of DNA, and can also remove 3′-phosphoglycolates (PGs) formed by free radical-mediated DNA cleavage.
Removal of 3'-phosphoglycolate from DNA strand-break …
WEBJan 1, 2001 — HAP1 was found to recognize the strand break, and catalyze the release of the 3'-phosphoglycolate as free phosphoglycolic acid. The enzyme had a Vmax of 0.1 fmole/min/pg of HAP1 protein, and a Km of 0.05 microM for …
Tyrosyl-DNA phosphodiesterase and the repair of 3′-phosphoglycolate ...
WEBTDP1 can also process the 3′-phosphoglycolate (3′-PG) moieties often formed during free radical-mediated DNA cleavage [4], as well as various other 3′ modifications [5], …
APE1 is the major 3'-phosphoglycolate activity in human cell
WEBJul 6, 2004 — DNA strand breaks containing 3'-phosphoglycolate (3'-PG) ends are the major lesions induced by ionizing radiation. The repair of this lesion is not completely understood and several activities are thought to be involved in processing of 3'-PG ends.
Processing of 3'-Phosphoglycolate-Terminated DNA Double …
WEBOct 1, 2005 — To assess the possibility that Artemis functions on oxidatively modified double-strand break termini, its activity toward model DNA substrates, bearing either 3{prime}-hydroxyl or 3{prime}-phosphoglycolate moieties, was examined.
WEBphosphoglycolate substrates, as well as a 3'-phosphate sub- strate, were resistant to DNase III under conditions in which the analogous 3'-hydroxyl substrates were extensively degrad-