MacConkey agar is commonly used to differentiate between the Enterobacteriaceae. Growth on MacConkey Agar with lactose fermentation Growth on MacConkey Agar without lactose fermentation ...

MacConkey agar is commonly used to differentiate between the Enterobacteriaceae. Organism on left is positive for lactose fermentation and that on the right is negative.

This image illustrates the specific morphological characteristics of three different organisms grown on MacConkey Agar. Top: Escherichia coli, Bottom left: Proteus vulgaris, Bottom right ...

The cases all pointed to the same suspect: agar. Agar is and has been a staple of microbiology labs for a century. “We buy it in bulk. We buy kilograms at a time,” Protacio told me.

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It is therefore important that any petri dish or agar used is sterilised before use, and that aseptic techniques are used to inoculate the plates. An inoculating loop can be used to transfer bacteria.